Recombinant Cadherin 16 Antibody / Rabbit Monoclonal

SKU: orb2640982

Description

The CDH16 gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. Cadherin 16 consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. [Wiki]

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human, Mouse
• Application Notes:
  The stated application concentrations are suggested starting points. Titration of the recombinant Cadherin 16 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: KSCP2-2R
• Immunogen: Human protein was used as the immunogen for the recombinant Cadherin 16 antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of human kidney lysate with recombinant Cadherin 16 antibody (clone KSCP2-2R). Expected molecular weight: 90~130 kDa.

IHC testing of FFPE human renal cell carcinoma with recombinant Cadherin 16 antibody (clone KSCP2-2R). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

IHC testing of FFPE mouse kidney tissue with recombinant Cadherin 16 antibody (clone KSCP2-2R). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

Quick Database Links

UniProt

O75309