Recombinant Cadherin 16 Antibody / Rabbit Monoclonal

SKU: orb386237

Description

Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that CDH16 (kidney-specific cadherin, Cadherin 16) is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, CDH16 is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. CDH16 has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

Research Area

Epigenetics

Images & Validation

• Tested Applications: FACS, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the recombinant Cadherin 16 antibody to be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: CDH16/1532R
• Immunogen: Human protein was used as the immunogen for the recombinant Cadherin 16 antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human renal cell carcinoma with recombinant Cadherin 16 antibody (clone CDH16/1532R). Required HIER: steam sections in 10 mM Tris with 1 mM EDTA, pH9.0, for 10-20 min.

IHC testing of FFPE mouse kidney tissue with recombinant Cadherin 16 antibody (clone CDH16/1532R). Required HIER: steam sections in 10 mM Tris with 1 mM EDTA, pH9.0, for 10-20 min.

IHC testing of FFPE rat kidney tissue with recombinant Cadherin 16 antibody (clone CDH16/1532R). Required HIER: steam sections in 10 mM Tris with 1 mM EDTA, pH9.0, for 10-20 min.

Western blot testing of human kidney lysate with recombinant Cadherin 16 antibody (clone CDH16/1532R). Expected molecular weight: 90~130 kDa.

Flow cytometry testing of human HEK293 cells with recombinant Cadherin 16 antibody (clone CDH16/1532R); Red = isotype control, Blue = recombinant Cadherin 16 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant Cadherin 16 antibody (clone CDH16/1532R) as confirmation of integrity and purity.

Quick Database Links

UniProt

O75309