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C14orf68 Antibody

Catalog Number: orb1247717

DispatchUsually dispatched within 5-10 working days
$ 880.00
Catalog Numberorb1247717
CategoryAntibodies
DescriptionC14orf68 Antibody
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IHC
ReactivityHuman
ImmunogenThe immunogen for this antibody is: C-KSRLQADGQGQRR
Antibody TypePrimary Antibody
Concentration500 ug/mL
Form/AppearanceLiquid
ConjugationUnconjugated
TargetC14orf68
UniProt IDQ6Q0C1
NCBINP_997000.2
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesC14orf68, chromosome 14 open reading frame 68 , HD
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NoteFor research use only
Application notesPeptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 22kDa band in Human Liver lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Liver shows staining of nucleoli in select hepatocytes. Recommended concentration, 2.5ug/ml.
Expiration Date12 months from date of receipt.
C14orf68 Antibody

orb1247717 (2.5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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