Goat anti-C14orf68 Antibody

• Catalog Number: orb19436
• Category: Antibodies
• Description: Goat polyclonal antibody to C14orf68
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Human
• Dilution range: ELISA: 1:16000, WB: 0.3 μg/ml, IHC-P: 2.5µg/ml
• Conjugation: Unconjugated
• MW: 33.4
• Target: C14orf68
• Entrez: 283600
• Protein Sequence: RYGNPDAKPTKAD
• RRID: AB_10926667
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti C14orf68 antibody, anti chromosome 14 open re
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Liver shows staining of nucleoli in select hepatocytes. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave an approx 45kDa band in Human Liver lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19436 (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.