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Catalog Number | orb385825 |
---|---|
Category | Antibodies |
Description | CD117, or p145kit, is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. CD117 antibody has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CLDA117 |
Tested applications | IHC |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant human protein was used as the immunogen for this CD117 antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This CD117 antibody is available for research use only. |
Entrez | 3815 |
Storage | Store the CD117 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD117 antibody to be titered up or down for optimal performance. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human Gastrointestinal stromal tumor using CD117 antibody
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