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Item 1 of 5
KIT Antibody (N-term D121)
Catalog Number: orb1939481
| Catalog Number | orb1939481 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB15297 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 109865 Da |
| Target | This KIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the N-terminal region of human KIT. |
| UniProt ID | P10721 |
| NCBI | NP_001087241.1, NP_000213.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Mast/stem cell growth factor receptor Kit, SCFR, P Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
KIT Antibody (N-term D121) western blot analysis in mouse testis tissue lysates (35 ug/lane). This demonstrates the KIT antibody detected the KIT protein (arrow).
Anti-KIT Antibody (N-term D121) at 1:1000 dilution + Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 110 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Flow cytometric analysis of NCI-H460 cells using KIT Antibody (N-term D121) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
KIT Antibody (N-term D121) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
KIT Antibody (N-term D121) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of KIT Antibody (N-term D121) for immunohistochemistry. Clinical relevance has not been evaluated.
