Butyrly-HIST1H3A (K9) Antibody

• Catalog Number: orb517066
• Category: Antibodies
• Description: This Butyrly-HIST1H3A (K9) Antibody is an unconjugated polyclonal product. It tagets HIST1H3A using a Peptide sequence around site of Butyrly-Lys (9) derived from Human Histone H3.1 as the immunogen. This antibody is suitable for ChIP, ELISA, IF, IHC, WB. Purification: Antigen Affinity Purified.
• Target: HIST1H3A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Purification: Antigen Affinity Purified
• Immunogen: Peptide sequence around site of Butyrly-Lys (9) derived from Human Histone H3.1
• UniProt ID: P68431
• Tested applications: ChIP, ELISA, IF, IHC, WB
• Dilution range: WB:1:500-1:5000, IHC-P:1:200-1:500, IF:1:50-1:200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3 histone family member E pseudogene antibody; H3
• Research Area: Epigenetics & Chromatin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- Butyrly-HIST1H3A (K9) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-

Western blot aanalysis of 293 whole cell lysate, A549 whole cell lysateusing Butyrly-HIST1H3A (K9) antibody

Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) using Butyrly-HIST1H3A (K9) antibody

Immunocytochemistry analysis of Hela cells using Butyrly-HIST1H3A (K9) antibody