Butyrly-HIST1H3A (K23) Antibody

• Catalog Number: orb517045
• Category: Antibodies
• Description: This Butyrly-HIST1H3A (K23) Antibody is an unconjugated polyclonal product. It tagets HIST1H3A using a Peptide sequence around site of Butyrly-Lys (23) derived from Human Histone H3.1 as the immunogen. This antibody is suitable for ChIP, ELISA, ICC, IF, IP, WB. Purification: Antigen Affinity Purified.
• Target: HIST1H3A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Purification: Antigen Affinity Purified
• Immunogen: Peptide sequence around site of Butyrly-Lys (23) derived from Human Histone H3.1
• UniProt ID: P68431
• Tested applications: ChIP, ELISA, ICC, IF, IP, WB
• Dilution range: WB:1:500-1:5000, IHC-P:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3 histone family member E pseudogene antibody; H3
• Research Area: Epigenetics & Chromatin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- Butyrly-HIST1H3A (K23) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real

Western blot aanalysis of Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysateusing Butyrly-HIST1H3A (K23) antibody

Immunoprecipitation analysis of Hela whole cell lysate using Butyrly-HIST1H3A (K23) antibody

Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) using Butyrly-HIST1H3A (K23) antibody

Immunocytochemistry analysis of Hela cells using Butyrly-HIST1H3A (K23) antibody