BTK Antibody

• Catalog Number: orb1239260
• Category: Antibodies
• Description: BTK Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Predicted Reactivity: Gallus
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: BTK antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK.The immunogen is located within amino acids 20 - 70 of BTK.
• Concentration: 1 mg/mL
• Dilution range: BTK antibody can be used for the detection of BTK by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 76 kDa Observed: 79 kDa
• Target: BTK
• UniProt ID: Q06187
• NCBI: Q06187
• Storage: BTK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: BTK Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: BTK Antibody: AT, ATK, BPK, XLA, IMD1, AGMX1, PSCT
• Note: For research use only
• Application notes: BTK antibody can be used for the detection of BTK by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BTK in U937 cell lysate with BTK antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.

Immunohistochemistry of BTK in mouse brain tissue with BTK antibody at 5 µg/mL.

Immunofluorescence of BTK in Daudi cells with BTK antibody at 10 µg/mL.

Immunocytochemistry of BTK in Daudi cells with BTK antibody at 10 µg/mL.

Immunofluorescence of BTK in mouse brain tissue with BTK antibody at 20 µg/ml. Red: BTK Antibody (orb1239260) Blue: DAPI staining.