Phospho-Lyn (Tyr507) (5B6) rabbit mAb

• Catalog Number: orb1946031
• Category: Antibodies
• Description: Phospho-Lyn (Tyr507) (5B6) rabbit mAb
• Clonality: Monoclonal
• Clone Number: LynY507-5B6
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr507 of human phospho Lyn
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P07948
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Tyrosine-protein kinase Lyn, Lck/Yes-related novel
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using Phospho-Lyn (Tyr507) antibody LynY507-5B6 at 0.01μg/mL. Cat. #orb1946031.

Peptide blocking flow cytometric analysis of Jurkat cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Lyn (Tyr507) antibody LynY507-5B6 at 0.01μg/mL. Cat. #orb1946031.

Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-Lyn (Tyr507) antibody LynY507-5B6 at 0.01μg/mL. Cat. #orb1946031.

Western blot analysis of extracts of HeLa cells treated with H2O2. Cell lysates were ran on a SDS-PAGE gel, transferred to nitrocellulose membrane, blocked and non-treated (-) or treated with lambda phosphatase (+) and stained using anti-phosph-Lyn (Tyr507) 5B6 rabbit recombinant antibody #orb1946031.

Western blot analysis of Hela cell extract, untreated or treated with H2O2 using Phospho-LYN (TYR507) antibody LYNY507-5B6 at 0.01 ug/mL. Cat. #orb1946031.

Western blot analysis of HeLa cell extract, untreated or treated with H2O2 using 0.01 μg/mL Phospho-Lyn (Tyr507) antibody LynY507-5B6 Cat. #orb1946031 or Company A antibody at 1 μg/mL (manufacturer's recommended concentration) developed using the same exposure.