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Catalog Number | orb1261228 |
---|---|
Category | Antibodies |
Description | BRCA1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide of human BRCA1 |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 300kDa |
Target | BRCA1 |
UniProt ID | P38398 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Breast cancer type 1 susceptibility protein, 632-, Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using BRCA1 antibody (orb1261228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human breast cancer using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using c-Jun antibody (orb1261262) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse pancreas using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
ChIP, ELISA, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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