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Rad51 Rabbit Polyclonal Antibody

Catalog Number: orb317801

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb317801
CategoryAntibodies
DescriptionRad51 Rabbit Polyclonal Antibody
TargetRAD51
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Equine, Porcine, Rabbit
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/PreservativesPreservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
ImmunogenKLH conjugated synthetic peptide derived from human Rad51 (81-180/339aa)
UniProt IDQ06609
MW37 kDa
Tested applicationsFC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:25, IF=1:100-500, Flow-Cyt=2ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesBRCA1/BRCA2 containing complex, subunit 5; BRCC 5;
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NoteFor research use only
Rad51 Rabbit Polyclonal Antibody

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-Rad51 antibody (orb317801), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Rad51 Rabbit Polyclonal Antibody

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Rad51) polyclonal Antibody, Unconjugated (orb317801) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Rad51 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Rad51) Polyclonal Antibody, Unconjugated (orb317801) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Rad51 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Rad51) Polyclonal Antibody, Unconjugated (orb317801) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Rad51 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Rad51) Polyclonal Antibody, Unconjugated (orb317801) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Rad51 Rabbit Polyclonal Antibody

Sample: bone (mouse) Lysate at 40 ug, Primary: Anti-Rad51 (orb317801) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37kD, Observed band size: 37kD.

Rad51 Rabbit Polyclonal Antibody

Sample: Hela (human) cell Lysate at 40 ug, Primary: Anti-Rad51 (orb317801) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37kD, Observed band size: 37kD.

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