ALB Antibody

SKU: orb2652822

Description

ALB Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Bovine
• Application Notes:
  Anti-Bovine Albumin antibody has been tested in ELISA and western blot and is suitable for IHC. The antibody may be suitable for other applications, although no supporting data is available. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 2.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Anti-Bovine Serum Albumin antibody was produced by repeated immunizations with Bovine Serum Albumin.
• Target: ALB
• Purity: Bovine Serum Albumin antibody is supplied in the form of monospecific antiserum prepared by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Albumin [Bovine Serum]. Cross reactivity against Albumin from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 85 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-Bovine Serum Albumin Antibody, Rabbit Anti-BSA, ALB antibody, Albumin antibody, Allergen Bos d 6 antibody, BSA antibody

Western Blot of Rabbit anti-Bovine Serum Albumin antibody. Lane 1: BSA (p/n orb2652777) Reduced [20 ng]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: BSA (p/n orb2652777) Non-Reduced [20 ng]. Primary antibody: Rabbit anti-BSA antibody at 1:10000 for overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG HRP MX10 secondary antibody (p/n orb347654) at 1:70000 for 30 min at RT. Block: 5% BLOTTO-TBS for 60 min at RT. Predicted/Observed size: 66 kDa, 66 kDa for BSA. Other band(s): None.

Western Blot of Rabbit anti-Bovine Serum Albumin antibody. Lane 1: BSA (p/n orb2652777). Load: 50 ng per lane. Primary antibody: BSA antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: 5% BLOTTO-TBS for 30 min at RT. Predicted/Observed size: 66 kDa, 66 kDa for BSA. Other band(s): None.

Quick Database Links

Gene Symbol

ALB

UniProt

P02769

RefSeq:CAA76847.1