ALB Antibody

SKU: orb2652804

Description

ALB Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution Range: ELISA: 1:120,000, IHC: 1:1,000 - 1:5,000, IP: 1:100, WB: 1:2,000 - 1:10,000
• Reactivity: Bovine
• Application Notes:
  Anti-Bovine Serum Albumin antibody has been tested by ELISA and Western Blot. The antibody may be suitable for other applications, although no supporting data is available

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 5F9
• Immunogen: Anti-Bovine Serum Albumin antibody was produced by repeated immunizations of balb/c mice with bovine serum albumin.
• Target: ALB
• Purity: Bovine Serum Albumin monoclonal antibody was purified from concentrated tissue culture supernate by Protein A chromatography and reacts with bovine serum albumin. The antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer. No cross reactivity is detected against other serum proteins by immunoblot. No reactivity is detected by ELISA against human serum albumin or ovalbumin.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.004 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-Bovine Serum Albumin Antibody, mouse anti-BSA Antibody, ALB antibody, Albumin antibody, Allergen Bos d 6 antibody, BSA antibody

ELISA Results of Mouse Anti-BSA Antibody. Each well was coated in duplicate with 10 µg of Bovine Serum Albumin. The working dilution is 1:85300. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Rabbit Anti-Mouse HRP conjugated (p/n orb347506) and TMB substrate (p/n orb348651).

Western Blot of Mouse anti-Bovine Serum Albumin Monoclonal Antibody. Lane 1: Bovine Serum Albumin. Load: 50 ng per lane. Primary antibody: Bovine Serum Albumin antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40000 for 30 min at RT. Block: 0.75% Casein for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Bovine Serum Albumin.

Quick Database Links

Gene Symbol

ALB

UniProt

P02769

RefSeq:AAA51411.1