Bovine IgG (H&L) Antibody Fluorescein Conjugated

SKU: orb346824

Description

Bovine IgG (H&L) antibody (FITC)

Images & Validation

• Tested Applications: DOT, FLISA, IF, WB
• Dilution Range: FLISA: 1:10,000 - 1:50,000, IF: 1:1,000 - 1:5,000
• Reactivity: Bovine
• Application Notes:
  Anti-Bovine IgG Fluorescein Conjugated Antibody has been tested by do blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Bovine IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Bovine IgG and Bovine Serum.
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000); Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti bovine IgG FITC, rabbit anti Bovine IgG Fluorescein Conjugated

Dot Blot results of Rabbit Anti-Bovine IgG Antibody Fluorescein Conjugated. Dots are Bovine IgG at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 60 min at RT. Primary Antibody: none. Secondary Antibody: Rabbit Anti-Bovine IgG Antibody Fluorescein at 1 µg/ml for 1hr at RT.