Bovine IgG F(ab')2 antibody

Catalog Number: orb346810

Catalog Number	orb346810
Category	Antibodies
Description	Bovine IgG F(ab')2 antibody
Species/Host	Goat
Clonality	Polyclonal
Tested applications	ELISA, IHC, WB
Reactivity	Bovine
Isotype	IgG
Immunogen	Bovine IgG F(ab')2 fragment
Concentration	5.0 mg/mL
Dilution range	ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Form/Appearance	Liquid (sterile filtered)
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Bovine IgG, Bovine IgG F(ab’)2 and Bovine Serum. No reaction was observed against Bovine IgG F(c).
Conjugation	Unconjugated
Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Alternative names	goat anti Bovine IgG F(ab')2 Antibody, goat anti-B Read more...
Note	For research use only
Application notes	Anti-Bovine IgG F(ab')2 antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Expiration Date	12 months from date of receipt.

Conjugation:	TRITC
Purity:	This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine IgG, anti-Bovine IgG F(ab’)2 and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(c) or anti-Pepsin.
Source:	Bovine

Sizes Available: 1 mg

Applications:	IP
Conjugation:	Agarose
Purity:	This product is purified Bovine IgG F(ab')2 isolated from normal Bovine Serum and then digested by the enzyme pepsin followed by subsequent chromatographic separation and coupling to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5.0 mg of anti-Bovine IgG.
Source:	Bovine

Sizes Available: 5 mg

Applications:	SDS-PAGE
Conjugation:	Unconjugated
Purity:	This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine IgG, anti-Bovine IgG F(ab’)2 and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(c) or anti-Pepsin.
Source:	Bovine

Sizes Available: 2 mg

Conjugation:	FITC
Purity:	This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Bovine IgG, anti-Bovine IgG F(ab’)2 and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(c) or anti-Pepsin.
Source:	Bovine

Sizes Available: 1 mg

Conjugation:	HRP
Purity:	This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Bovine IgG, anti-Bovine IgG F(ab’)2 and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(c) or anti-Pepsin.
Source:	Bovine

Sizes Available: 1 mg