Bovine IgG F(ab')2 Antibody Fluorescein Conjugated

SKU: orb346811

Description

Bovine IgG F(ab')2 antibody (FITC)

Images & Validation

• Tested Applications: DOT, FC, FLISA, IF
• Dilution Range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Reactivity: Bovine
• Application Notes:
  Anti-Bovine IgG F(ab')2 Fluorescein Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Bovine IgG F(ab')2 fragment
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Bovine IgG, Bovine IgG F(ab’)2 and Bovine Serum. No reaction was observed against Bovine IgG F(c).
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000); Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Bovine IgG F(ab')2 fragment fluorescein Conjugated Antibody, goat anti-Bovine IgG Fab2 fragment FITC Conjugated Antibody

Dot Blot of Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated. Antigen: Bovine IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated at 1:1000 for 1 HR at RT. Block: orb348637 for 1 HR at RT.