Bovine IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated

SKU: orb346832

Description

Bovine IgG F(ab')2 antibody (Alkaline Phosphatase)

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:20,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Reactivity: Bovine
• Application Notes:
  Anti-Bovine IgG F(ab’)2 Alkaline Phosphatase Conjugate has been tested by ELISA and dot blot. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,600 to 1:11,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Bovine IgG F(ab')2 fragment
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Bovine IgG, Bovine IgG F(ab’)2 and Bovine Serum. No reaction was observed against Bovine IgG F(c).
• Conjugation: AP

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
• Concentration: 0.6 mg/mL
• Disclaimer: For research use only

Alternative Names

rabbit anti-Bovine IgG F(ab')2 alkaline phosphatase Conjugated Antibody, rabbit anti-Bovine IgG Fab2 alk phos Conjugated Antibody

ELISA Results of Purified Rabbit Anti-Bovine IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated tested against purified Bovine IgG F(ab')2 Alk Phos. Each well was coated in duplicate with 1.0 µg of Bovine IgG F(ab')2 (p/n orb2652778). The working dilution is 1:15200. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase ELISA Substrate and NPP Working Buffer.