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Catalog Number | orb346547 |
---|---|
Category | Antibodies |
Description | Bovine IgG F(ab')2 antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine |
Isotype | IgG |
Immunogen | Bovine IgG F(ab')2 fragment |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Lyophilized |
Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Bovine IgG, Bovine IgG F(ab’)2 and Bovine Serum. No reaction was observed against Bovine IgG F(c). |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Alternative names | goat anti-Bovine IgG F(ab')2 fragment Antibody, go Read more... |
Note | For research use only |
Application notes | BOVINE IgG F(ab')2 fragment antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |
TRITC | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine IgG, anti-Bovine IgG F(ab’)2 and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(c) or anti-Pepsin. | |
Bovine |
IP | |
Agarose | |
This product is purified Bovine IgG F(ab')2 isolated from normal Bovine Serum and then digested by the enzyme pepsin followed by subsequent chromatographic separation and coupling to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5.0 mg of anti-Bovine IgG. | |
Bovine |
SDS-PAGE | |
Unconjugated | |
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine IgG, anti-Bovine IgG F(ab’)2 and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(c) or anti-Pepsin. | |
Bovine |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Bovine IgG, anti-Bovine IgG F(ab’)2 and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(c) or anti-Pepsin. | |
Bovine |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Bovine IgG, anti-Bovine IgG F(ab’)2 and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(c) or anti-Pepsin. | |
Bovine |
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