BOLL Antibody

SKU: orb1247727

Description

BOLL Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 22kDa band in Human Testis lysates after 1.5 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.6kDa according to NP_932074.1 and 31.3kDa according to NP_149019.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. However, literature reports a 36kDa band in Human and Mouse Testes (PMID: 11390979 ). Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Testis shows cytoplasm staining in spermatocytes, but not in spermatogonia. Recommended concentration, 4-6µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QPEPIKTVWSIHY
• Target: BOLL
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BOLL, bol, boule-like (Drosophila), BOULE, boule

Quick Database Links

Gene Symbol

BOLL

UniProt

Q8N9W6

RefSeq (Protein):NP_932074.1, NP_149019.1