BOULE/BOLL Antibody

SKU: orb18976

Description

Goat polyclonal antibody to BOLL

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 1.5 μg/ml, IHC-P: 4-6µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 22kDa band in Human Testis lysates after 1.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.6kDa according to NP_932074.1 and 31.3kDa according to NP_149019.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. However, literature reports a 36kDa band in Human and Mouse Testes (PMID: 11390979 ). Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: BOULE / BOLL
• Molecular Weight: 32.6; 31.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti BOLL antibody, anti bol, boule-like (Drosophila) antibody, anti BOULE antibody, anti boule antibody

Immunohistochemical staining of Human Testis using BOLL antibody

Quick Database Links

Gene Symbol

BOULE / BOLL

NCBI Gene ID

66037