BOULE/BOLL Antibody

• Catalog Number: orb18976
• Category: Antibodies
• Description: Goat polyclonal antibody to BOLL
• Target: BOULE / BOLL
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QPEPIKTVWSIHY
• RRID: AB_10767254
• MW: 32.6; 31.3
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 1.5 μg/ml, IHC-P: 4-6µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 22kDa band in Human Testis lysates after 1.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.6kDa according to NP_932074.1 and 31.3kDa according to NP_149019.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. However, literature reports a 36kDa band in Human and Mouse Testes (PMID: 11390979 ). Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti BOLL antibody, anti bol, boule-like (Drosophi
• Note: For research use only
• Entrez: 66037
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Testis using BOLL antibody