BLMH Antibody (Center)

• Catalog Number: orb1927287
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 52562 Da
• Target: This BLMH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-242 amino acids from the Central region of human BLMH.
• UniProt ID: Q13867
• NCBI: NP_000377.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Bleomycin hydrolase, BH, BLM hydrolase, BMH, BLMH
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

BLMH Antibody (Center) western blot analysis in K562 cell line, mouse stomach and rat stomach tissue lysates (35 ug/lane).This demonstrates the BLMH antibody detected the BLMH protein (arrow).

Immunohistochemical analysis of paraffin-embedded H. skin section using BLMH Antibody (Center). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes: Anti-BLMH Antibody (Center) at 1:1000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lane 3: Neuro-2a whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 53 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-BLMH Antibody (Center) at 1:1000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: CCRF-CEM whole cell lysate. Lane 3: SH-SY5Y whole cell lysate. Lane 4: MOLT-4 whole cell lysate. Lane 5: Neuro-2a whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 53 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.