BLMH Antibody (Center)

SKU: orb1927287

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology, Neuroscience

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:25
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This BLMH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-242 amino acids from the Central region of human BLMH. Antigen Region: 212-242 aa.
• Target: BLMH
• Molecular Weight: 52562 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bleomycin hydrolase, BH, BLM hydrolase, BMH, BLMH

BLMH Antibody (Center) western blot analysis in K562 cell line, mouse stomach and rat stomach tissue lysates (35 ug/lane).This demonstrates the BLMH antibody detected the BLMH protein (arrow).

Immunohistochemical analysis of paraffin-embedded H. skin section using BLMH Antibody (Center). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes: Anti-BLMH Antibody (Center) at 1:1000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lane 3: Neuro-2a whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 53 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-BLMH Antibody (Center) at 1:1000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: CCRF-CEM whole cell lysate. Lane 3: SH-SY5Y whole cell lysate. Lane 4: MOLT-4 whole cell lysate. Lane 5: Neuro-2a whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 53 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

BLMH

UniProt

Q13867

RefSeq (Protein):NP_000377.1