BLMH Antibody (Center)

• Catalog Number: orb1788288
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Predicted Reactivity: Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: 212-242 aa
• Dilution range: IF: 1:25, WB: 1:1000, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 52562 Da
• Target: This BLMH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-242 amino acids from the Central region of human BLMH.
• UniProt ID: Q13867
• NCBI: NP_000377.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: BLMH;Bleomycin hydrolase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

BLMH Antibody (Center) western blot analysis in CEM, HL-60 cell line lysates (35 ug/lane). This demonstrates the BLMH antibody detected the BLMH protein (arrow).

Western blot analysis of lysates from K562 cell line, mouse stomach tissue lysate (from left to right), using BLMH Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Fluorescent image of Hela cells stained with BLMH Antibody (Center). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).