Biotin Monoclonal Antibody

SKU: orb344396

Description

Biotin antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA: 1:20,000, FC: 1-2 µg/mL, IHC: 1:1,000 - 1:5,000, IF: 2 ug/mL, WB: 1:2,000 - 1:10,000
• Application Notes:
  Anti-Biotin antibody has been tested in ELISA, western blot and immunofluorescence and is suitable for lateral flow, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 4C7.D5
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
• Purity: Biotin Antibody was prepared from mouse ascites by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store Biotin antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-Biotin Monoclonal Antibody, Anti-Biotin, Vitamin B4 antibody