Biotin Monoclonal Antibody

SKU: orb344394

Description

Biotin antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA: 1:20,000, FC: 1-2 µg/mL, IHC: 1:1,000 - 1:5,000, IF: 2 ug/mL, WB: 1:2,000 - 1:10,000
• Application Notes:
  Anti-Biotin antibody has been tested in ELISA, western blot and immunofluorescence and is suitable for lateral flow, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 4C7.D5
• Immunogen: Anti-Biotin Antibody was produced by repeated immunizations with Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH).
• Purity: Anti-Biotin Antibody was prepared from mouse ascites by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store Anti-Biotin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-Biotin Monoclonal Antibody, Biotin Antibody, Vitamin H, Coenzyme R