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Catalog Number | orb766531 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Tubulin beta |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Bovine, Fish, Human, Mouse, Porcine, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human Tubulin beta. AA range:401-450 |
Concentration | 1 mg/ml |
Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
Conjugation | Unconjugated |
MW | 55 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-TUBB3 antibody, anti-TUBB4 antibody, anti-Tub Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.
Immunofluorescence analysis of human-uterus tissue. 1, Tubulin β Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Immunofluorescence analysis of rat-kidney tissue. 1, Tubulin β Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Western Blot analysis of various cells using Tubulin β Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000.
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |