lacZ Antibody

SKU: orb344788

Description

Beta galactosidase antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:10,000, IHC: 1:1,500, WB: 1:5,000 - 1:10,000
• Application Notes:
  Anti-Beta-Gal Antibody has been tested by ELISA and western blot and is suitable for dot blot, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 µm) to produce free-floating sections.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Full length native Beta Galactosidase isolated from E.coli
• Target: lacZ
• Purity: Beta-Galactosidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.coli]. Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Beta Galactosidase Antibody, rabbit anti-beta gal antibody, ß-Gal, Anti-ß-Gal Antibody

Quick Database Links

Gene Symbol

lacZ

UniProt

P00722

RefSeq (Protein):NP_414878.1