Beta galactosidase antibody

• Catalog Number: orb344789
• Category: Antibodies
• Description: Beta galactosidase antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Isotype: IgG
• Immunogen: Full length native Beta Galactosidase isolated from E.coli
• Concentration: 1.0mg/mL
• Dilution range: ELISA: 1:10,000, IHC: 1:1,500, IF: User Optimized, WB: 1:5,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Beta-Galactosidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.coli]. Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.
• Conjugation: Unconjugated
• UniProt ID: P00722
• NCBI: NP_414878.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Beta Galactosidase Antibody, rabbit an
• Note: For research use only
• Application notes: Anti-Beta-Gal Antibody has been tested by ELISA and western blot and is suitable for dot blot, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 µm) to produce free-floating sections.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of shows detection of a band at ~117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead) using beta GALACTOSIDASE antibody

Western blot analysis of (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate us

Western blot analysis of Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel using beta GALACTOSIDASE antibody