b-Catenin Antibody

SKU: orb385954

Description

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. [RefSeq]

Research Area

Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways, Wnt

Images & Validation

• Tested Applications: FACS, IF, WB
• Dilution Range: Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml
• Reactivity: Human
• Application Notes:
  Titration of the b-Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: 9F2
• Immunogen: Chicken b-Catenin was used as the immunogen for the b-Catenin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human 1) A431, 2) A549 and 3) MCF7 cell lysate with Beta Catenin antibody (clone 9F2). Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

SDS-PAGE Analysis of Purified, BSA-Free b-Catenin Antibody (clone 9F2). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P35222