ctnnb1 Antibody

SKU: orb345707

Description

beta Catenin antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:25000, IHC: 5 - 10 ug/mL, WB: 1:500
• Reactivity: Human, Zebrafish
• Application Notes:
  beta catenin antibody has been tested for use in ELISA, western blotting, immunohistochemistry, and ISH. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 85.5 kDa in size corresponding to catenin beta-1 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: beta catenin antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to catenin beta-1 C-terminus.
• Target: ctnnb1
• Purity: beta catenin antibody is directed against catenin beta-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with catenin beta-1 protein from human, mouse, rat, bovine, Xenopus laevis, and Danio rerio at 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.15 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-beta Catenin Antibody, rabbit anti-Catenin beta antibody, catenin beta-1, ß-catenin-1, ß-catenin, CTNNB1, CTNNB, beta-catenin antibody

Quick Database Links

Gene Symbol

ctnnb1

UniProt

F1QGH7

RefSeq (Protein):NP_001091679.1