ctnnb1 Antibody

SKU: orb345706

Description

beta Catenin antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:75,000 - 1:125,000, IHC: 5 - 10 ug/mL, WB: 1:500
• Reactivity: Human, Zebrafish
• Application Notes:
  beta catenin antibody has been tested for use in ELISA, western blotting, immunohistochemistry, and ISH. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 85.5 kDa in size corresponding to catenin beta-1 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: beta catenin antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to catenin beta-1 C-terminus.
• Target: ctnnb1
• Purity: beta catenin antibody is directed against catenin beta-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with catenin beta-1 protein from human, mouse, rat, bovine, Xenopus laevis, and Danio rerio at 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-beta Catenin Antibody, rabbit anti-Catenin beta antibody, catenin beta-1, ß-catenin-1, ß-catenin, CTNNB1, CTNNB, beta-catenin antibody

Quick Database Links

Gene Symbol

ctnnb1

UniProt

F1QGH7

RefSeq (Protein):NP_001091679.1