beta Catenin Antibody

SKU: orb345706

Description

beta Catenin antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:75,000 - 1:125,000, IHC: 5 - 10 ug/mL, WB: 1:500
• Reactivity: Human, Zebrafish
• Application Notes:
  beta catenin antibody has been tested for use in ELISA, western blotting, immunohistochemistry, and ISH. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 85.5 kDa in size corresponding to catenin beta-1 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: beta catenin antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to catenin beta-1 C-terminus.
• Purity: beta catenin antibody is directed against catenin beta-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with catenin beta-1 protein from human, mouse, rat, bovine, Xenopus laevis, and Danio rerio at 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-beta Catenin Antibody, rabbit anti-Catenin beta antibody, catenin beta-1, ß-catenin-1, ß-catenin, CTNNB1, CTNNB, beta-catenin antibody

Immunohistochemistry of Rabbit anti-beta Catenin antibody. Tissue: human small intestine epithelium. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: beta Catenin antibody at 5-10 µg/ml for 1 h at RT. Secondary antibody: Peroxidase goat anti-rabbit at 1:10000 for 45 min at RT. Localization: Strong membranous staining in a variety of epithelial tissues. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Western Blot of Rabbit anti-Beta Catenin antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate (p/n orb348669). Lane 2: HeLa Lysate (p/n orb348667). Lane 3: MCF-7 Lysate (p/n orb348664). Lane 4: Jurkat Lysate. Lane 5: A431 Lysate (p/n orb348665). Lane 6: A549 Lysate (p/n orb348675). Lane 7: LNCap Lysate (p/n orb348694). Lane 8: MOLT-4 Lysate (p/n orb348696). Lane 9: Ramos Lysate. Lane 10: Raji Lsyate (p/n orb348672). Lane 11: A-172 Lysate (p/n orb348708). Lane 12: NIH/3T3 Lysate (p/n orb348714). Load: 10 µg per lane. Primary antibody: Beta catenin antibody at 1:1000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:30000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 85 kDa for Beta Catenin.

Western Blot of Rabbit anti-catenin ß-1 antibody. Lane 1: zebrafish embryos injected with myc tagged catenin ß 1 mRNA. Lane 2: zebrafish embryos injected with myc tagged catenin ß 2 mRNA. Lane 3: zebrafish embryos un-injected. Primary antibody: catenin ß-1 antibody at 1:500 overnight at 4°C. Secondary antibody: goat anti-rabbit HRP at 1:10000 for 1 hour at RT. Predicted/Observed size: 85.5kDa/ ~125kDa (arrow) endogenous catenin ß-1. Other band(s): ~110kDa (star) co-migrating catenin ß-1 and ß-2.

Quick Database Links

UniProt

F1QGH7

RefSeq (Protein):NP_001091679.1