beta Catenin Antibody, KO Validated

• Catalog Number: orb1274536
• Category: Antibodies
• Description: beta Catenin Antibody, KO Validated
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human beta Catenin (NP_001895.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 105kDa
• Target: CTNNB1
• UniProt ID: P35222
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: CTNNB1, CTNNB, MRD19, armadillo
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using beta Catenin antibody (orb1274536) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western blot analysis of extracts from normal (control) and beta Catenin knockout (KO) HeLa cells, using beta Catenin antibody (orb1274536) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human tonsil using beta Catenin antibody (orb1274536) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human mammary cancer using beta Catenin antibody (orb1274536) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of U-87MG cells, using 3 ug beta Catenin antibody (orb1274536). Western blot was performed from the immunoprecipitate using beta Catenin antibody (orb1274536) at a dilution of 1:1000.