ACTB Antibody

SKU: orb420225

Description

beta Actin antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:5000-1:50000, WB: 1 µg/mL
• Reactivity: Human, Mouse
• Application Notes:
  Anti-beta Actin antibody has been tested for use in ELISA, IHC and Western Blot. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 3H2.E4.E12
• Immunogen: Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with ß-Actin peptide near the N-Terminus followed by hybridoma development.
• Target: ACTB
• Purity: Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. beta Actin antibody is specific for human and mouse ß Actin protein. Expected to cross-react with a wide range of species due to sequence homology. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates. Cross-reactivity with other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

Quick Database Links

Gene Symbol

ACTB

UniProt

P60709

RefSeq (Protein):NP_001092.1