ACTB Antibody

SKU: orb420224

Description

beta Actin antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:5000-1:50000, WB: 1 µg/mL
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Anti-beta Actin antibody has been tested for use in ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 3H2.E4.E12
• Immunogen: Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with ß-Actin peptide near the N-Terminus followed by hybridoma development.
• Target: ACTB
• Purity: Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. beta Actin antibody is specific for human and mouse ß Actin protein. Expected to cross-react with a wide range of species due to sequence homology (human, mouse, rat, swine). A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates. Cross-reactivity with other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

Immunohistochemistry of Mouse anti-Beta Actin N-Term antibody. Tissue: human spleen. Fixation: formalin fixed paraffin archival tissues. Antigen retrieval: not required. Primary antibody: Beta Actin N-Term antibody at 2.5 µg/ml for 1 h at RT. Secondary antibody: Peroxidase goat anti-rabbit at 1:10000 for 45 min at RT. Localization: membranous and cytoplasmic. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Western Blot of Mouse anti-beta Actin antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate (p/n orb348669). Lane 2: HeLa Lysate (p/n orb348668). Lane 3: MCF-7 Lysate (p/n orb348664). Lane 4: Jurkat Lysate. Lane 5: A431 Lysate (p/n orb348665). Lane 6: A549 Lysate (p/n orb348675). Lane 7: LNCap Lysate (p/n orb348694). Lane 8: MOLT-4 Lysate (p/n orb348696). Lane 9: Raji Lysate (p/n orb348672). Lane 10: Ramos Lysate. Lane 11: PC-12 (p/n orb348733). Load: 10 µg per lane. Primary antibody: beta-Actin antibody at 1:1000 o/n at 4C. Secondary antibody: Peroxidase mouse secondary (p/n orb347506) at 1:40000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 42 kDa for beta-Actin.

Western Blot of Mouse anti-N-Term Beta Actin Monoclonal Antibody. Lane 1: HeLa Whole Cell Lysate (p/n orb348668). Lane 2: NIH/3T3 Whole Cell Lysate (p/n orb348714). Load: 10 µg per lane. Primary antibody: N-Term Beta Actin Monoclonal Antibody at 1.13 µg/ml overnight at 4°C. Secondary antibody: HRP mouse secondary antibody (p/n orb347506) at 1:40000 for 30 min at RT. Block: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 42 kDa, 42 kDa for N-Term Beta Actin.

Quick Database Links

Gene Symbol

ACTB

UniProt

P60709

RefSeq (Protein):NP_001092.1

Yisheng Chen 1, Zhongcheng Fan 2, Zhiwen Luo 1, Xueran Kang 3, Renwen Wan 1, Fangqi Li 1, Weiwei Lin 4, Zhihua Han 5, Beijie Qi 1, Jinrong Lin 1, Yaying Sun 1, Jiebin Huang 6, Yuzhen Xu 7, Shiyi Chen Impacts of Nutlin-3a and exercise on murine double minute 2-enriched glioma treatment Neural Regen Res, (2024)

PubMed: 38989952

Applications

WB

Reactivity

Mouse