beta Actin antibody

• Catalog Number: orb420224
• Category: Antibodies
• Description: beta Actin antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3H2.E4.E12
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2a
• Immunogen: Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with ß-Actin peptide near the N-Terminus followed by hybridoma development.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5000-1:50000, IHC: User Optimized, WB: 1 µg/mL
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. beta Actin antibody is specific for human and mouse ß Actin protein. Expected to cross-react with a wide range of species due to sequence homology (human, mouse, rat, swine). A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates. Cross-reactivity with other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P60709
• NCBI: NP_001092.1
• Storage: Store Anti-Beta Actin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-beta Actin Antibody, Actin Antibody, Lo
• Note: For research use only
• Application notes: Anti-beta Actin antibody has been tested for use in ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.