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Description
Research Area
Cancer Biology
Images & Validation
−
Item 1 of 3
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:5000-1:50000, WB: 1 µg/mL |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | 3H2.E4.E12 |
| Immunogen | Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with ß-Actin peptide near the N-Terminus followed by hybridoma development. |
| Target | ACTB |
| Purity | Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. beta Actin antibody is specific for human and mouse ß Actin protein. Expected to cross-react with a wide range of species due to sequence homology (human, mouse, rat, swine). A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates. Cross-reactivity with other sources has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−mouse anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001092.1 |
|---|
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Filter by Applications
Filter by Species
Yisheng Chen 1, Zhongcheng Fan 2, Zhiwen Luo 1, Xueran Kang 3, Renwen Wan 1, Fangqi Li 1, Weiwei Lin 4, Zhihua Han 5, Beijie Qi 1, Jinrong Lin 1, Yaying Sun 1, Jiebin Huang 6, Yuzhen Xu 7, Shiyi Chen Impacts of Nutlin-3a and exercise on murine double minute 2-enriched glioma treatment Neural Regen Res, (2024)
Applications
WB
Reactivity
Mouse