F-Actin Rabbit Polyclonal Antibody

SKU: orb10068

Description

F-Actin Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3μg /test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic MAP peptide derived from human beta-Actin (1-50/374aa)
• Target: ACTB
• Molecular Weight: 42 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ACTB_HUMAN; Actin, cytoplasmic 1; ACTB; EC:3.6.4.- ; Beta actin; Actin, cytoplasmic 1, N-terminally processed; actin beta; BNS; CSMH; DDS1; THC8; BKRNS; BRWS1; PS1TP5BP1; Actin cytoplasmic 1; β actin; β-actin; βactin;

Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-F-Actin antibody (orb10068), Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/test. Protocol, The cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (orb10068) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (orb10068) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Hela (Human) Whole Cell Lysate at 30 ug, Lane 2: Jurkat (Human) Whole Cell Lysate at 30 ug, Lane 3: 293T (Human) Whole Cell Lysate at 30 ug, Lane 4: Hepg2 (Human) Whole Cell Lysate at 30 ug, Lane 5: A431 (Human) Whole Cell Lysate at 30 ug, Lane 6: A549 (Human) Whole Cell Lysate at 30 ug, Lane 7: H9C2 (Rat) Whole Cell Lysate at 30 ug, Lane 8: Testis (Mouse) Tissue Lysate at 30 ug, Primary: Anti-F-Actin (orb10068) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.

Quick Database Links

Gene Symbol

ACTB

UniProt

P60709

References

Ying Zhong # 1, Hui Kang # 1, Ziqing Ma # 1, Jiayu Li 1, Zixi Qin 1, Zixuan Zhang 1, Peiwen Li 1, Ying Zhong 1, Lihui Wang Vasorin Exocytosed from Glioma Cells Facilitates Angiogenesis via VEGFR2/AKT Signaling Pathway Mol Cancer Res ., (2024)

PubMed: 38488456

Applications

IHC

Reactivity

Mouse

Y Guo, Y Wang, Q Li, Q Liu, X Zhang, J Ren, C Wang Bisphenol A disrupts the neuronal F-actin cytoskeleton by activating the RhoA/ROCK/LIMK pathway in Neuro-2a cells Toxicology, (2024)

PubMed:

Applications

WB