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Featured
Description
Research Area
Cancer Biology, Cell Biology
Images & Validation
−
Item 1 of 4
| Tested Applications | ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1/5000-1/10000 IHC: 1/50-1/200 IF: 1/100-1/200 |
| Reactivity | Canine, Gallus, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | B32H3X5C5X5D3 |
| Immunogen | Synthetic Peptide of β-actin |
| Target | ACTB |
| Molecular Weight | Calculated MW: 42 kDa; Observed MW: 42 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Customer Validated Data
− The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution
Latest Experiments
9 ResultsDilution
Sample
Summary
WB
Human, Rat
1:5000
Protein lysates and cell lysates
This antibody was used as a...
WB
Mouse
-
Protein lysates from RAW264.7 cells and mouse colon tissue
This antibody was used as a...
WB
Human
-
Protein lysates and HEK 293 Cells
This antibody was used as a...
WB
Mouse
1:3000
Protein lysates from mouse lung tissue
This antibody was used as a...
WB
Chicken
-
Protein lysates from chick jejunum tissue
This antibody was used as a...
Previous
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Buffer/Preservatives | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta
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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Quick Database Links
Gene Symbol
ACTB
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
Available Sizes
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