Bestatin

SKU: orb1708791

Description

Bestatin

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research

Key Properties

• CAS Number: 58970-76-6
• MW: 308.37
• Purity: 99.54%
• Formula: C₁₆H₂₄N₂O₄
• SMILES: C([C@H]([C@@H](C(N[C@@H](CC(C)C)C(O)=O)=O)O)N)C1=CC=CC=C1
• Target: Antibiotic,Aminopeptidase,Antibacterial,LTR
• Solubility: 10% DMSO+40% PEG300+5% Tween-80+45% Saline:0.5 mg/mL (1.62 mM);1eq. NaOH:15.4 mg/mL (49.94 mM);DMSO:10 mg/mL (32.43 mM)

Bioactivity

• In Vivo:
  Bestatin exhibits a concentration dependent Inhibition of SN12M cell invasion into reconstituted basement membrane (matrigel). It also concentration dependently inhibit the degradation of type IV collagen by tumor cells in a non-neoplastic condition medium. In U937 cells, bestatin enhance the activity of caspase-3, inducing DNA laddering and fragmentation. Additionally, in SN12M, bestatin inhibit the hydrolytic activity of aminopeptidase substratees. furthermore , bestatin inhibits tube formation in human umbilical vein endothelial cells. Through binding to leucine aminopeptidase fixed o the cell surfacee, bestatin directly stimulates lymphocytes and monocytes), whereas it indirectly does so by impedin the degradation metabolism o the phagocytosis stimulating hormones throug the Inhibition of aminopeptidase B.
• In Vitro:
  In mice bearing B16-BL6 melanoma tumors on their dorsolateral sides, Bestatin reduce the number of blood vessels in established Primary tumor masses. Additionally, Bestatin significantly inhibited angiogenesis induced by melanoma cells in the dorsal air sac assay in mice. In EGDA rats, Bestatin decrease the incidence of EAC from 57.7% to 26.1%. Moreover, Bestatin demonstrated statistically significant Inhibition of leukotriene B4 biosynthesis in the esophageal tissues of EGDA rats.
• Cell Research:
  Growing cells ( × 06 to × 06 cells/mL) are diluted to 1. × 03 cells mL and transferred (3 mL) into a well in a 12-well multiwell plate (2.5 cm diameter/well). Cells are treated with 0, 10, 50, 100, 300, or 600 μM Bestatin and allowed to grow at 21°C shaking at 180 rpm for 48 h. A hemocytometer is used to measure cell density after 0, 24, and 48 h.

Storage & Handling

• Storage: Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Bestatin, Bacterial, APN, Antibiotic, Aminopeptidase, inhibit, Inhibitor, LTA(4)H-h, Ubenimex

Compound Identifiers

PubChem CID

72172