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Catalog Number | orb377134 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody against Beclin 1 conjugated to APC |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC |
Reactivity | Human, Rat |
Immunogen | Synthetic peptide from the C-terminus of human Beclin 1 |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000); ICC/IF (1:100) |
Conjugation | APC |
MW | 51kDa |
Target | Beclin 1 |
Entrez | 8678 |
UniProt ID | Q14457 |
NCBI | NP_001300927.1 |
Storage | Conjugated antibodies should be stored according to the product label |
Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
Alternative names | APG6 antibody, BCL-2 interacting protein beclin an Read more... |
Note | For research use only |
Application notes | A 1:1000 dilution of SPC-601 was sufficient for detection of Beclin1 on 293T lysates using Goat anti-rabbit IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 1 Polyclonal Antibody. Tissue: SK-N-BE. Species: Human. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody at 1:200 for Overnight. Secondary Antibody: Anti-Rabbit: AlexaFluor 555 at 1:1000. Counterstain: Hoechst, Phalloidin AlexaFluor 647 at 1:1000. Localization: Mitochondria, endosomes/exosomes. A) Hoechst Nuclear Stain B) Phalloidin AlexaFluor 647 C) Anti-Beclin 1 D) Merge.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 1 Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Golgi apparatus, Dendrites and Cell bodies of cerebellar Purkinje cells. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 1 Antibody (D) Composite.
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 μg). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.
Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human 293T (20 μg). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.
IF | |
Bovine, Porcine | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
APC |
IF | |
Canine, Equine, Gallus, Human, Mouse, Rabbit | |
Rat | |
Rabbit | |
Polyclonal | |
APC |