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Rubicon Antibody: APC
Catalog Number: orb55258
| Catalog Number | orb55258 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody against Rubicon conjugated to APC. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ICC, IF, IHC |
| Reactivity | Human |
| Immunogen | Synthetic peptide from the N-terminal of Human Rubicon |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000), ICC/IF (1:100); IHC (1:50) |
| Conjugation | APC |
| MW | 95 kDa |
| Target | Rubicon |
| Entrez | 9711 |
| UniProt ID | Q92622 |
| NCBI | NP_001139114.1 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
| Alternative names | Run domain Beclin-1-interacting and cysteine-rich Read more... |
| Note | For research use only |
| Application notes | A 1:1000 dilution of SPC-668 was sufficient for detection of Rubicon in 15 µg of human Hela cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.
Immunohistochemistry analysis using Rabbit Anti-Rubicon Polyclonal Antibody. Tissue: Tonsil. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~108 kDa Rubicon protein using Rabbit Anti-Rubicon Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~108 kDa.
