BDNF Antibody

• Catalog Number: orb1248588
• Category: Antibodies
• Description: BDNF Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF
• Predicted Reactivity: Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: ETKCNPMGYTKE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:2000.Immunofluorescence: Expression of the protein seen in the nucleus and cytoplasm of Glioblastoma U251 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: BDNF
• UniProt ID: P23560
• NCBI: NP_733931.1, NP_733928.1, NP_733929.1, NP_001700.2, NP_733930.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: BDNF , brain-derived neurotrophic factor, MGC34632
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:2000.Immunofluorescence: Expression of the protein seen in the nucleus and cytoplasm of Glioblastoma U251 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248588 (1 ug/ml) staining of PFA-perfused cryosection of Mouse sciatic nerve (left) and injured sciatic nerve (right). Alexa 488-staining.

orb1248588 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing speckled nuclear and cytoplasmic staining.

orb1248588 (5 ug/ml) staining of paraffin embedded Rat Cerebral Cortex (A) and Rat Hippocampus (B), CRY-staining with DAPI nuclear counter stain in blue. Antigen retrieval with citrate buffer pH 6 at 95-98C.

orb1248588 (5 ug/ml) staining of paraffin embedded Rat Cerebral Cortex. Antigen retrieval with citrate buffer pH 6 at 95-98C, DAB-staining. This data is from a previous batch, not on sale.

orb1248588 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.