BDNF Antibody

SKU: orb1248588

Description

BDNF Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:2000.Immunofluorescence: Expression of the protein seen in the nucleus and cytoplasm of Glioblastoma U251 cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: ETKCNPMGYTKE
• Target: BDNF
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BDNF , brain-derived neurotrophic factor, MGC34632, neurotrophin

orb1248588 (1 ug/ml) staining of PFA-perfused cryosection of Mouse sciatic nerve (left) and injured sciatic nerve (right). Alexa 488-staining.

orb1248588 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing speckled nuclear and cytoplasmic staining.

orb1248588 (5 ug/ml) staining of paraffin embedded Rat Cerebral Cortex (A) and Rat Hippocampus (B), CRY-staining with DAPI nuclear counter stain in blue. Antigen retrieval with citrate buffer pH 6 at 95-98C.

orb1248588 (5 ug/ml) staining of paraffin embedded Rat Cerebral Cortex. Antigen retrieval with citrate buffer pH 6 at 95-98C, DAB-staining. This data is from a previous batch, not on sale.

orb1248588 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

Quick Database Links

Gene Symbol

BDNF

UniProt

P23560

RefSeq (Protein):NP_001700.2, NP_733929.1, NP_733931.1, NP_733928.1, NP_733930.1