Trk A + B + C Recombinant Rabbit Monoclonal Antibody

SKU: orb612228

Description

Trk A + B + C Recombinant Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200, ICC/IF=1:50-200
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 5T6
• Immunogen: KLH conjugated synthetic peptide derived from human Trk-B : 800-822/822
• Target: NTRK2
• Molecular Weight: 90 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Pan Trk; TrkA/B/C; TrkA+B+C; Trk A; Trk B; Trk C; TrkB; TrkC; Tyrosine Receptor Kinase A; Tyrosine Receptor Kinase B; Tyrosine Receptor Kinase C.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min, Antibody incubation with Trk A + B + C Monoclonal Antibody, Unconjugated (orb612228) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Sealing solution and Concentration: 5% NFDM/TBST dilution of primary antibody: 1:2000, Incubation conditions for primary antibody: 2 hours at room temperature, Secondary antibody: Goat Anti Rabbit IgG H&L (HRP), Cracking solution: 1: Mouse brain, 2: Rat brain, Protein loading amount: 20 μg, Exposure time: 60 seconds, Theoretical molecular weight: 92 kDa, Actual molecular weight: 120-140 kDa.

Quick Database Links

Gene Symbol

NTRK2

UniProt

Q16620