DEFB103B Antibody

SKU: orb1272731

Description

DEFB103B Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  ELISA:Indirect:To detect hBD-3 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-3.SandwichTo detect hBD-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human BD-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-3. Western Blot:To detect hBD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-3. Human BD-3 specific antibody was purified by affinity chromatography employing immobilized hBD-3 matrix.
• Target: DEFB103B
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BD-3, HBD3, HBP3, DEFB3, DEFB103, BD3, DEFB103BBeta-defensin 103, Beta-defensin 3, BD-3

To detect hBD-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BD-3 (orb1272730) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-3.

Quick Database Links

Gene Symbol

DEFB103B

UniProt

P81534

RefSeq:P81534