Beta Defensin 3 Rabbit Polyclonal Antibody (FITC)

SKU: orb187581

Description

Beta Defensin 3 Rabbit Polyclonal Antibody (FITC) is an FITC conjugated antibody targeting DEFB103A. This antibody is suitable for FC. It exhibits reactivity with Human samples.

Research Area

Defensins, Immune System, Inflammation, Macrophage

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1μg/Test
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human beta Defensin 3 (23-67/67aa)
• Target: DEFB103A
• Purification: Affinity purified by Protein A
• Conjugation: FITC

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

BD 3; BD-3; BD3; beta 103; beta defensin 103A; beta defensin 103A precursor; beta defensin 3; beta-defensin 103; beta-defensin 3; D103A_HUMAN; DEF B3; DEFB 103; DEFB 103A; DEFB 3; DEFB-3; DEFB103; DEFB103A; DEFB103B; DEFB3; Defensin; Defensin beta 103A; Defensin beta 3; Defensin like protein; Defensin-like protein; HBD 3; hBD-3; HBD3; HBP 3; HBP3; mBD3.

Blank control (blue): Hep G2 Cells (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-beta Defensin 3/FITC Conjugated antibody (orb187581), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC (orange), used under the same conditions.

Paraformaldehyde-fixed, paraffin embedded (Human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-beta Defensin 3 Antibody, conjugated (orb187581) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-beta Defensin 3 Antibody, conjugated (orb187581) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

DEFB103A