BCL9 Antibody

SKU: orb1254374

Description

BCL9 Antibody

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein of mouse Bcl9
• Target: BCL9
• Molecular Weight: Observed: 160kDa/170kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

2610202E01Rik, 8030475K17Rik, A330041G23Rik, Gm13B-cell CLL/lymphoma 9 protein, B-cell lymphoma 9 protein, bcl-9, nuclear co-factor of beta-catenin signalling

Western blot analysis of extracts of various cell lines, using Bcl9 antibody (orb1254374) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat brain using BCL9 antibody (orb1254374) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using BCL9 antibody (orb1254374) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

BCL9