BCL9L Antibody

• Catalog Number: orb1239054
• Category: Antibodies
• Description: BCL9L Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: Bcl9L antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human Bcl9L.The immunogen is located within amino acids 20 - 70 of Bcl9L.
• Concentration: 1 mg/mL
• Dilution range: Bcl9L antibody can be used for detection of Bcl9L by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 165 kDa Observed: 195 kDa
• Target: BCL9L
• UniProt ID: Q86UU0
• NCBI: NP_872363
• Storage: Bcl9L antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Bcl9L Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Bcl9L Antibody: BCL9-2, DLNB11, Protein BCL9-2, B-
• Note: For research use only
• Application notes: Bcl9L antibody can be used for detection of Bcl9L by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Bcl9L in HeLa cell lysate with Bcl9L antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry of Bcl9L in HeLa cells with Bcl9L antibody at 10 µg/mL.

Immunofluorescence of Bcl9L in human breast tissue with Bcl9L antibody at 20 µg/ml. Green: Bcl9L Antibody (orb1239054) Blue: DAPI staining.

Immunohistochemistry of Bcl9L in human breast tissue with Bcl9L antibody at 5 µg/ml.