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| Catalog Number | orb1239054 |
|---|---|
| Category | Antibodies |
| Description | Bcl9L Antibody |
| Target | BCL9L |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 1 mg/mL |
| Buffer/Preservatives | Bcl9L Antibody is supplied in PBS containing 0.02% sodium azide. |
| Purification | Bcl9L Antibody is affinity chromatography purified via peptide column. |
| Immunogen | Bcl9L antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human Bcl9L.The immunogen is located within amino acids 20 - 70 of Bcl9L. |
| UniProt ID | Q86UU0 |
| MW | Predicted: 165 kDa Observed: 195 kDa |
| Tested applications | ELISA, ICC, IF, IHC-P, WB |
| Application notes | Bcl9L antibody can be used for detection of Bcl9L by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Bcl9L Antibody: BCL9-2, DLNB11, Protein BCL9-2, B- Read more... |
| Research Area | Cancer Biology, Cell Biology |
| Note | For research use only |
| NCBI | NP_872363 |

Western blot analysis of Bcl9L in HeLa cell lysate with Bcl9L antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry of Bcl9L in HeLa cells with Bcl9L antibody at 10 µg/mL.

Immunofluorescence of Bcl9L in human breast tissue with Bcl9L antibody at 20 µg/ml. Green: Bcl9L Antibody (orb1239054) Blue: DAPI staining.

Immunohistochemistry of Bcl9L in human breast tissue with Bcl9L antibody at 5 µg/ml.
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Human | |
0.32-20 ng/mL | |
0.121 ng/mL |
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