Bcl6 Antibody

• Catalog Number: orb750093
• Category: Antibodies
• Description: Antibody to Bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show Bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, Bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (L&H) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BCL6/1475
• Tested applications: WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Amino acids 256-389 were used as the immunogen for the Bcl6 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 0.5-1ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Bcl6 antibody is available for research use only.
• UniProt ID: P41182
• Storage: Store the Bcl6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Bcl6 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Protein array validation of the Bcl6 antibody: Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Bcl6 antibody (clone BCL6/1475). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Western blot testing of 1) partial recombinant protein and 2) human HepG2 lysate with Bcl6 antibody (clone BCL6/1475). Expected molecular weight: 78/95 kDa (non-phosphorylated/phosphorylated).

SDS-PAGE analysis of purified, BSA-free Bcl6 antibody (clone BCL6/1475). Confirmation of integrity and purity of the antibody.