Bcl6 Antibody

• Catalog Number: orb2635141
• Category: Antibodies
• Description: Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ( LH ) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human protein was used as the immunogen for the Bcl6 antibody.
• UniProt ID: P41182
• Tested applications: FACS, IF
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Application notes: Optimal dilution of the Bcl6 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PCRP-BCL6-1E2
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Bcl6 antibody is available for research use only.
• Note: For research use only

Immunofluorescent staining of human MCF-7 cells using Bcl6 antibody (green, clone PCRP-BCL6-1E2) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with Bcl6 antibody (blue, clone PCRP-BCL6-1E2), and unstained cells (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Bcl6 antibody (clone PCRP-BCL6-1E2). These results demonstrate the foremost specificity of the PCRP-BCL6-1E2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.