BCL2 Antibody

SKU: orb1331720

Description

BCL2 Antibody

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB 1:2000, IHC 1:150
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Immunogen: Full length human recombinant protein of human BCL2 (NP_000624) produced in E.coli.
• Target: BCL2
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bcl-2; PPP1R50

Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-BCL2 monoclonal antibody (1:500).

Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-BCL2 monoclonal antibody (1:200).

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BCL2 mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BCL2 mouse monoclonal antibody.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2 (RC204498, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.

Quick Database Links

Gene Symbol

BCL2