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Catalog Number | orb1251866 |
---|---|
Category | Antibodies |
Description | Bcl10 Antibody [SPM520] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | SPM520 |
Tested applications | FC, IF, IHC, WB |
Reactivity | Human |
Isotype | IgG1, kappa |
Immunogen | Amino acids 122-168 were used as the immunogen for this Bcl10 antibody. |
Antibody Type | Primary Antibody |
Concentration | 0.2 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | BCL10 |
UniProt ID | O95999 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Bcl-10 Antibody: CLAP, mE10, CIPER, c-E10, CARMEN, Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The optimal dilution of the Bcl10 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human tonsil stained with Bcl10 antibody (SPM520).
FC, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |