CARD11 Antibody

Catalog Number: orb18487

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Product Properties

Catalog Number	orb18487
Category	Antibodies
Description	Goat polyclonal antibody to CARD11
Target	CARD11
Clonality	Polyclonal
Species/Host	Goat
Conjugation	Unconjugated
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Protein Sequence	QRKTIWVDEDQL
RRID	AB_10750722
MW	133
Tested applications	ELISA, IHC, WB
Dilution range	ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 2-5 μg/ml
Application notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining. Recommended concentration, 2-5ug/ml.WB: Approx 150+65kDa bands observed in lysates of cell line Jurkat (calculated MW of 133kDa according to NP_115791.2). A similar pattern was observed in HEK293 transiently expressing CARD11 (pciture not shown). These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative names	anti CARD11 antibody, member 11 antibody, anti BIM Read more...
Research Area	Cancer, Epigenetics
Note	For research use only
Entrez	84433